ࡱ> lnk#` R2bjbjmm6V 2222222F&&&&LZ&FH2''''''''ZH\H\H\H\H\H\H$JhLPH!2'''''H22''H 2 2 2'2'2'ZH 2'ZH 2 2C22E'& ޴&{0zD.F,H0HDL1L,EL2E'' 2'''''HH1'''H''''FFFJFFFJFFF222222  0;Sf[RP[uirf[[ 0 zYef['Y~ q\N'Yf[;Sf[b 0;Sf[RP[uirf[[ 0 z[Yef['Y~ z T y ;Sf[RP[uirf[[ e T y Practice of Medical Molecular Biology z  S [ '` ( TRe'`[ z  # N Sfq _>e[yvpe 'Y ~ ;N d N NS RGY NBhpg _ _O>N тS 'Y ~ [ 8h N тS N0f[e0f[R z;`f[e 40 [f[e40 z;`f[R [f[R N0(uNNSt^~ W@x04N^020ST;Sf[0btf[0of[NNvN0mQ0Nt^6R N0[Yef[vvNW,gBl Ǐ;Sf[RP[uirf[T'`[Yef[ \~ހuirf[0uirSf[NRP[uirf[0;Sf[W Of[f[yvW@xtwƋ0[bd\O0f[yvMRlۏU\NSuuvRP[:gtSluSRI{wƋۏLR_~0T b_b|~SvwƋ~g0Ǐ[Yef[ Of[uq`[[v8^(uNhV W{Qf[uRKbR0Ss v^NUSrf[ywƋSO|[ۏL~TRg N ㉳Q0ǏDeg0;`~ f[u]~bQ v^[ۏLSc"} W{Qf[uvyxR0ReaƋSV|^y0 V0;NNhVY NO)nؚy_:g0+}YSRIQIQ^0R`)n4ltm{0gIQPn>f_\0uirf[>f_\0vhN0DNA_ϑKm[N0CO2R`)nu[{05ulN05ulN0Wv5ulňn0QbPN0PCRꁨRp_sN0+}Y\N0_ϑR7hhV0g4YSy_{0[hVv0R`)n4ltm{I{ N0[ zQ[Tf[eRM ^ S[yv T y[ Q [f[eRM[Blc[ Ye^]_/*g_1ˆ}v(pSb/gkhKm`Tck8^N@n-NvMQutˆ}v1 ˆ}v7hTYt\`Nck8^N@nR+RReQ N7hQm l4ltm5min 2 SDS-PAGEv6RYHQM6RRy QMnSm) 3 ˆ}v N7h 5ulRy@n-N N T~Rvˆ}v( 4 lǑ(u5uly\ˆ}vNly]xx~~ }(NC) N0\4gxgg0wm~0n~0Q0NC0n~0wm~03gxggO!k>e}Y 9Y}Y9YP[ >eeQ5uli-N 180mA ly2h 5 MQuf[hKmǑ(u9hǏ'lSirvHRP hvbSOhKmvvˆ}v0SQNC!51vY|\1h!ReQNbS^1h!PBSmm3!k k!k10min!ReQHRPhvNbS^1h!PBSmm3!k k!k10min!ReQ>fr^irDABTvvˆ}vsS>f:yQhĞr0Ǐag&^rvmEmT gekck8^NT`@n-NMQuMQutˆ}vvh]_8R\~ۏL_Nt^6R-N]_ NmQt^6R-N*g_U\02PCRb/gStS^(u 1)(uTrizolՋBR[\ ~ހۏL*2:HJ^r8   , 2 4 H öyytoe^oRotIh#CJ\o(hh\aJo( h#CJo(hc5CJ\o( ho( h#o(h5CJ\o(h#5CJ\o(hch#5CJ\aJo(hch5CJ\aJo(hJ^5CJ\aJhhJ^5CJ.\o(hJ^5CJ.\o(hJ^5CJ^\o(hJ^hJ^5CJV\o(hhJ^5CJ\aJo(hJ^5CJ\aJo(*,.02468:rt: |  dhgd  $dha$gdJ^ $da$gdJ^$d88XDdYDda$gdJ^$d88XDdYDda$gdJ^2 6 8 H  @ B ^  2 dhWD`gd  dh`gd   dhWD` gd $a$$dhWD`a$gd  dhWD`gd  $dha$gd dhgd   . 2 4 6 8 : @ \ ^ n   $ : > B F J      ( . 0 B D N P ^ ` ȾþþþþþùùҰҰssss"h#B*KHOJQJaJo(phh#B*KHOJQJaJph"hd;B*KHOJQJaJo(phhd;5CJo(h#5CJo( hd;o( hP2eo( h#o( h:io( ho( h#CJo(hCJ\o(h#CJ\o(h#5CJ\o(h5CJ\o(-` 02ltvĹҫ{oaT{JC h aJo(h 5CJ\o(hs^h#5CJ\o(h#5CJH*\aJo(h#5CJ\aJo( h#CJo(h#5CJ\o(hd;5CJ\o(h 5CJo(hd;h#5CJo(hclPh;OJQJaJo(h;OJQJaJo(hd;B*OJQJo(phh;B*OJQJo(phh#B*OJQJo(ph"hd;B*KHOJQJaJo(ph26:DRblv| $$Ifa$gds^$If $$Ifa$ ) $$Ifa$gdT kd$$Ifl4?֞@9!$   h H H    0$4 lalf4 |~*****(*髡|pgeppUhyvOJQJo(h hyvOJQJo(h hyvOJQJ hUVo( hyvo(h hyv5OJQJaJo(hyv5CJ\o( h CJo( hU5o(h 5CJ\o(h h 5\aJo(h h OJQJaJh h OJQJaJo( h o( h aJo(hjh aJo(%P4~ $IfgdT  $$Ifa$gdT  $Ifgd  ) $$Ifa$gdyvkd#$$Ifl4?֞@9!$   h H H    0$4 lalf4*+++++ ,,, $Ifgdyvi$IfVD2WDd^i`gdyv $$Ifa$gdUV$;$7$8$G$H$IfWD`;a$gdUV $$Ifa$gdyv ;`RNAvcSNt[ 2)lU_S^;`RNA 500ng0Random hexaprimer,AMV reverse transcriptase Buffer0AMV reverse transcriptase0RNase inhibitor0dNTP 3)PCRibXvvWVibX\ hvuVP[0_ir:Nyr_'`_ir ibX^:N442bpQ[gq:N-actin vQibX^218bp0PCRibX 4)PCRS^~gvhKm f_\ N‰[R~ހQYvR^`Q032R\~ۏL _O>N*g_ mQ08he_ ~T[d\OT[bJTnx[b~0 (***,*>*@*b*d*******++ + +++"+6+8+>+J+R+T+Z+^+`+h+v+z+++++++++++++++++++++ŗŐňh hyvo( h(b9hyvhDAhyvo( h BaJo(h[hyvaJo( hyvaJo(h(b9hyvaJ h Bo(h(b9hyvo( hUVo( hyvo(h BOJQJh hyvOJQJh hyvOJQJo(h BOJQJo(3++ ,,,,,,>,@,,,,,,,,,"-$-L-N-`-f-r-x-z---------... ...0.^.`.....ؾӷ| hBaJo(h.OnhB5CJ\o(hfhBQJo( haQJo( hBQJo( hBo(hB5CJ\o( hU5CJo( hU5aJo(hU5OJQJo( hao( hU5o(hU55CJ\o( hyvCJo( hyvo( hyvaJo(hyv5CJ\o(.,,,<,) $$Ifa$gdU5kdF$$Ifl4?֞@9!$   h H H    0$4 lalf4<,~,,, -J-b-f-r-z-- $$Ifa$gdU5 $IfgdU5 $IfgdU5 ---) $$Ifa$gdBkdi$$Ifl4?֞@9!$   h H H    0$4 lalf4---.,.\...... $$Ifa$gdB $Ifgda $IfgdB $IfgdB ........./B/J/L/P/R/X/Z/`/b/f/h/j/l/ùӃyrkaUI?hG,hzCJo(hG,hz\aJo(hG,hG,\aJo(hG,h;.iaJo( h;.iaJo( hzaJo(hG,hG,aJo(hz5CJ\aJo(!hahz5OJQJ\aJo(hzOJQJaJo(hahzOJQJaJo(h}uhzaJo(h}uhzB*\aJo(phhz5CJ\o( hBCJo(hB5CJ\o( hBo(hB5CJ\aJo(...) $$Ifa$kd$$Ifl4?֞@9!$   h H H    0$4 lalf4.... /L/P/R/Z/b/h/ $$Ifa$$$IfVD^a$gdz $$Ifa$gda$Z$IfVDWD+^`Za$gda$If h/j/l/) $$Ifa$kd$$Ifl4?֞@9!$   h H H    0$4 lalf4l/n/////000 $$Ifa$ $$Ifa$gdz $$Ifa$gdsX$Ifl/n///////////0000 0,04080:0<0>0@0^0`0b0f0x0z0000000001¶}s}i}}}}}}}i}ih;.ih6>aJo(h;.ihBaJo(h;.ihG,aJo(h;.ihG,aJh;.ihG,QJaJhB5CJ\o(hG,5CJ\o( hzCJo(hz5CJ\aJo( hG,aJo(hz5CJ\o(hzhzOJQJaJo(hahzOJQJaJo(hzOJQJaJo( hzo(&00 0) $$Ifa$kd$$Ifl4?֞@9!$ h H H    0$4 lalf4 0:0b00P1|111111 $$Ifa$ $$Ifa$gd6> $Ifgd6>$dh$IfWDd`a$gd;.i$dh$Ifa$gd6>$If 1$1&1P11111111111111112{tjth#5CJ\o( h#CJo( hBCJo(h;.ihB\aJo(h;.ih;.i\aJo(hB5CJ\aJo( h;.iaJo(hB5CJ\o(h;.i5CJ\o(h;.ihB5\aJo(h;.ih6>aJh;.ih6>aJo(h;.ih6>H*OJQJaJo(h;.ih6>OJQJaJo(1111)''kd $$Ifl4?֞@9!$   h H H    0$4 lalf412gdUV4&P 182P. A!Q"Q#n$n%Sn !$$Ifl!vh555h5H5H55#v#v#vh#vH#v:V l4?0$,555h5H5/  /  /  4alf4!$$Ifl!vh555h5H5H55#v#v#vh#vH#v:V l4?0$,555h5H5/  /  /  4alf4!$$Ifl!vh555h5H5H55#v#v#vh#vH#v:V l4?0$,555h5H5/  /  /  4alf4!$$Ifl!vh555h5H5H55#v#v#vh#vH#v:V l4?0$,555h5H5/  /  /  4alf4!$$Ifl!vh555h5H5H55#v#v#vh#vH#v:V l4?0$,555h5H5/  /  /  4alf4+$$Ifl!vh555h5H5H55#v#v#vh#vH#v:V l4?0$++,555h5H5/  /  /  4alf4+$$Ifl!vh555h5H5H55#v#v#vh#vH#v:V l4?0$++,555h5H5/  /  /  4alf4!$$Ifl!vh555h5H5H55#v#v#vh#vH#v:V l4?0$,555h5H5/  /  /  4alf4N@N cke $1$a$$CJKHPJ_HaJmH nHsH tH$A@$ ؞k=W[SOFi@F nfhAGHJe   >Tu-DFLPSTVej}0o 0000000000000000000000000000000 00 0 0 0 00 0 0 0 0 00000 0 0 0 0 0 0 00 000 0 0  0 0 0 0 000000 0 0 0 0 0 0 0 00000 0 0 0 0 0 0 0 00 0 0 00 0 0 0 0 000 0 0 0 0 0 0 0 0@000 0 0 0 0 0 0009:i $Svw   ")16;>AGHJe   >Tu-DFLPSTVej}0o 00000000@00~00|000000000000000000000000000000000 00 0 0 0 00 0 0 0 0 00000 0 0 0 0 0 0 00 00000 0 0 0 0 0 0 0 000000 0 0 0 0 0 0 0 00000 0 0 0 0 0 0 0 0000 0 0 0 0 0 0 0 000 0 0 0 0 0 0 0 00000 0 0 0 0 0 000 ` (*+.l/12  %( 2,<,--..h/l/0 0112 !"#$&')*2 8@0(  B S  ?%8:;=>@ACEHQijlmoprt    #$*AFSXptw !"#'()*+,-./0156:;=CDFJdg,0>@ACJMSY[\`fhkrxz{  &4QRklq{| .29<=@ILSVilnqtw,/CFKLOPRVdeil|.069FNdimnqy{  % >@yOSnr 33333s33s3%i ;>@HJTV %>@ n \:]#!P\*, \>81쯘B|kEP\/xDcҬJ Go~( hh^h`OJQJo( H\H^H`\hH) \^`\hH. \^`\hH. 4\4^4`\hH)  \ ^ `\hH. | \| ^| `\hH.  \ ^ `\hH) \^`\hH.  ^` 5o(0H\H^H`\)\^`\.\^`\.4\4^4`\) \ ^ `\.| \| ^| `\. \ ^ `\)\^`\.hh^h`o(H\H^H`\)\^`\.\^`\.4\4^4`\) \ ^ `\.| \| ^| `\. \ ^ `\)\^`\.hh^h`B*CJOJQJo(phH\H^H`\)\^`\.\^`\.4\4^4`\) \ ^ `\.| \| ^| `\. \ ^ `\)\^`\. \^`\5o(0H\H^H`\)\^`\.\^`\.4\4^4`\) \ ^ `\.| \| ^| `\. \ ^ `\)\^`\.  ^` 5o(0H\H^H`\)\^`\.\^`\.4\4^4`\) \ ^ `\.| \| ^| `\. \ ^ `\)\^`\.z^`zCJo(H\H^H`\)\^`\.\^`\.4\4^4`\) \ ^ `\.| \| ^| `\. \ ^ `\)\^`\. \^`\o(0 t0t^t`0o( \^`\.\^`\.4\4^4`\) \ ^ `\.| \| ^| `\. \ ^ `\)\^`\.GoB]#!kE*,/xDc81n 6        0$         0        G        "IT         ?        _j :k       T s^o +G,U56>R ? BsXP2e!i;.i:i}u/{yvaB UV;#d;J^a+zc )16;AGHJe  DFLPSTVe QU@f 2 PP*UnknownGz Times New Roman5Symbol3& z Arial;[SOSimSun?5 z Courier New 1hʦ@ԦxƦYY!-!),.:;?]}    & 6"0000 0 0 00000 =@\]^([{  0 0 00000;[Qnid f2qHX ?!i2 [Yef['Y~Q?@ABCDEFGHIJKLMNOPQRSTUVWXYZ\]^_`abdefghijmRoot Entry FR޴oData ,1Table4LWordDocument6VSummaryInformation([DocumentSummaryInformation8cCompObjm  FMicrosoft Office Word ĵ MSWordDocWord.Document.89q